Description
nZ
Horse
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Silver Dilution is a dominant trait. This means,
in order to inherit the trait, a horse requires only one parent
to carry and pass on the gene. Somewhat similar to the Agouti gene,
the Silver Dilution gene will only alter black pigmented horses
(Ee or EE) and has no effect on red pigmented horses (ee). The Agouti
gene alters the coat by controlling distribution of the black pigment
whereas the Silver Dilution gene does so by diluting areas of black
pigment.
The effects of the Silver Dilution gene can vary greatly. Dilution
by the Silver gene on a horse with a uniform black base typically
involves lightening of the mane and tail and a dilution of the body
to a chocolate color, often dappled as well. A Bay horse carrying
the Silver gene will usually have a lightened mane and tail, as
well as lightened lower legs. It is important to know that although
a red horse will not be diluted by the Silver gene, it can however
be a carrier of the gene and thus potentially pass the gene on to
its offspring.
Silver dilution has been identified in a number of horse breeds
including the Quarter horse, the Rocky Mountain horse, the Icelandic
horse, Morgans, Shetland ponies and the Miniature horse.
Reference:
Brunberg E, Andersson L, Cothran G, Sandberg K, Mikko
S and Lindgren G. 2006. A
missense mutation in PMEL17 is associated with the Silver coat color
in the horse. BMC Genetics 7:46
Silver Dilution
Testing
Animal Genetics offers
DNA testing and detection of the gene mutations responsible for
Silver Dilution.
Why Test For Silver Dilution?
There are a number of reasons testing for the
silver dilution gene can be beneficial. 1) Since the Silver Dilution
gene only dilutes black pigment and does not physically alter red
pigmented horses, testing red based horses will identify carriers
of the Silver gene. 2) For Silver horses and carriers of the gene,
testing to determine zygosity (whether the horse has one or two
copies of the gene) can help with color breeding programs. 3) In
some situations, the Silver gene can cause a coat color that might
look very similar to another. For example, Silver Dilution on a
Buckskin might resemble a Palomino or, another example, some Silver
Bays and Silver Dapples have been incorrectly referred to as Chocolate
Palominos. Testing can help determine the actual color of the horse.
Cost
$25.00
US per sample.
Sample Collection
Collect sample by pulling (not cutting) 20-30
mane or tail hairs with roots attached. It is important that you
pull the hairs and confirm that the actual root of the hair is being
collected. The root contains the genetic material of your horse
that is needed for DNA testing. Therefore, cut hairs do not provide
an adequate sample of your horse. Place the collected hairs of each
horse in a separate zip-lock bag labeling the bags accordingly with
the horses name or identification number. Download and complete
a submission form for each sample and send along with
payment to Animal Genetics for testing.
Results
Results are given using the following symbolic
notation:
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ZZ
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Horse tested Homozygous
for Silver Dilution (Two copies of the Silver allele detected).
Black-based horses will have a chocolate body with flaxen mane
and tail. Bay-based horses will have lightened lower legs and
flaxen mane and tail. |
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nZ
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Horse tested Heterozygous
for Silver Dilution (One copy of the Silver allele detected).
Black-based horses will have a chocolate body with flaxen mane
and tail. Bay-based horses will have lightened lower legs and
flaxen mane and tail. |
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nn
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Horse tested
negative for Silver Dilution. |
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